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Require further optimization. In addition to viral vectors, recombinant bacterial carriersRequire further optimization. In addition to viral vectors, recombinant bacterial carriers, derived from lactic acid bacteria, Salmonella and L. monocytogenes strains, have been used extensively as delivery systems being able to stimulate both systemic [29,30] and mucosal immune responses .GENE DELIVERY
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Require further optimization. In addition to viral vectors, recombinant bacterial carriersRequire further optimization. In addition to viral vectors, recombinant bacterial carriers, derived from lactic acid bacteria, Salmonella and L. monocytogenes strains, have been used extensively as delivery systems being able to stimulate both systemic [29,30] and mucosal immune responses .GENE DELIVERY
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Ata have been very first preprocessed, removing SNPs using a GC score.7, and these that had been not genotyped in all participants. SNPs with minor allele frequency.1 and these significantly diverging from Hardy einberg equilibrium have been also removed. The remaining SNPs had been filtered to contain only those present inside the metabolic/ protein rotein interaction (PPI) network used withi
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RpoDhs-lacZ)) (27), which is a MC4100 derivative that lacks the rpoH gene but is able to grow at 37 due to the overproduction of GroEL and GroES, was used for the purification of 32. AD202 (MC4100 ompT::kan) (28) was used for the purification of FtsH. HB101 (supE (mcrC-mrr) recA ara-14 proA lacY galK rpsL xyl-5 mtl-1 leuB thi-1) and MC4100 were used for the purification of DnaK and DnaJ, respect
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[21] has not been so encouraging . the antigenic/therapeutic protein in vivo[21] has not been so encouraging . the antigenic/therapeutic protein in vivo and to stimulate [25] potent specific humoral and cellular immune responses . RNA viral vectors, such as retrovirus and lentivirus, allow long-term expression of the transgene, while DNA viral vectors allow expression in episomal form. Viral
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