Ged recombinant proteins of Tric1 and Tric2 were produced by Gateway cloning into pDEST17 and expressed in BL21 cells. Tric1SAM was expressed and purified as described above. The equal abundance of recombinant proteins was confirmed by detection with 63 His antiserum (Qiagen).Fluorescein-Labeled RNA OligonucleotidesBinding analysis was performed using 59-fluorescein-labeled RNA oligonucleotides (S
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