1
Is promoted by higher temperatures (10). In addition to proteases, the DnaK chaperone system consisting of DnaK (Hsp70), DnaJ (Hsp40), and GrpE (nucleotide exchange factor) is required for the rapid degradation of 32 in vivo because the half-life of 32 is much longer in dnaK, dnaJ, and grpE mutants (11, 12). 32 directly interacts with DnaK or DnaJ and forms a stable ternary complex in the presence