Primers used to create the WT and mutant 95?8 kcnj2 recombinants are2. Materials and Methods2.1. Bioinformatic Analysis of Zebrafish kcnj2 Gene Orthologues. A manual reciprocal best hit approach was undertaken [22]. The human KCNJ2 amino acid sequence was queried against the zebrafish genome using the TBLASTN algorithm on Ensembl Genome Browser (Zv9 build), with search sensitivity settings on "dis