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Rboxylterminus. The amplicon was subsequently introduced into the pGEM T-Easy vector (Promega) according to the manufacturer's instructions, and the products were bidirectionally sequenced to confirm their identity. The QuikChange SiteDirected Mutagenesis Kit (Stratagene) protocol was used to perform in vitro site-directed mutagenesis to create the 95?98 kcnj2 construct. The primers used in this p