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MM HEPES-KOH, pH 7.4, 75 mM NaCl, and 10 glycerol). Anisotropy measurements (excitation wavelength, 494 nm; emission wavelength, 524 nm) were recorded at 22 using a FluoroLog spectrofluorometer (Horiba Scientific) following successive additions of purified Tric1SAM (wild type or variants; concentration ranging from 0 to 1,350 nM). The binding reactions were allowed to stabilize for 200 s before
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MM HEPES-KOH, pH 7.4, 75 mM NaCl, and 10 glycerol). Anisotropy measurements (excitation wavelength, 494 nm; emission wavelength, 524 nm) were recorded at 22 using a FluoroLog spectrofluorometer (Horiba Scientific) following successive additions of purified Tric1SAM (wild type or variants; concentration ranging from 0 to 1,350 nM). The binding reactions were allowed to stabilize for 200 s before
1
MM HEPES-KOH, pH 7.4, 75 mM NaCl, and 10 glycerol). Anisotropy measurements (excitation wavelength, 494 nm; emission wavelength, 524 nm) were recorded at 22 using a FluoroLog spectrofluorometer (Horiba Scientific) following successive additions of purified Tric1SAM (wild type or variants; concentration ranging from 0 to 1,350 nM). The binding reactions were allowed to stabilize for 200 s before
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Were incubated with 80 mM purified TricSAM (wild type or variants) in a binding Plant Physiol. Vol. 172,Murcha et al.Supplemental Figure S3. Tric1 and Tric2 interactions with subunits of the protein import apparatus. Supplemental Figure S4. Characterization of single and double T-DNA insertion knockouts for Tric1 and Tric2. Supplemental Figure S5. tRNA import assays into mitochondria isolated from
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Were incubated with 80 mM purified TricSAM (wild type or variants) in a binding Plant Physiol. Vol. 172,Murcha et al.Supplemental Figure S3. Tric1 and Tric2 interactions with subunits of the protein import apparatus. Supplemental Figure S4. Characterization of single and double T-DNA insertion knockouts for Tric1 and Tric2. Supplemental Figure S5. tRNA import assays into mitochondria isolated from
1
Were incubated with 80 mM purified TricSAM (wild type or variants) in a binding Plant Physiol. Vol. 172,Murcha et al.Supplemental Figure S3. Tric1 and Tric2 interactions with subunits of the protein import apparatus. Supplemental Figure S4. Characterization of single and double T-DNA insertion knockouts for Tric1 and Tric2. Supplemental Figure S5. tRNA import assays into mitochondria isolated from
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Iative under the following accession numbers: Tric1 (At3g49560), Tric2 (At5g24650), Tim23-2 (At1g72750), Tim17-2 (At2g37410), Tim22 (At3g10110/ At1g18320), RISP (At5g13430), MPP-a (At3g16480), and tRNAAla (At3g62245). Sequencing data are available from the National Center for Biotechnology Information Gene Expression Omnibus database under accession number GSE76287.Supplemental DataThe following s
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Iative under the following accession numbers: Tric1 (At3g49560), Tric2 (At5g24650), Tim23-2 (At1g72750), Tim17-2 (At2g37410), Tim22 (At3g10110/ At1g18320), RISP (At5g13430), MPP-a (At3g16480), and tRNAAla (At3g62245). Sequencing data are available from the National Center for Biotechnology Information Gene Expression Omnibus database under accession number GSE76287.Supplemental DataThe following s