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Turret1gong

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TochemistryA miRIDIAN mimic for miR-634 (C-300961-01), a scrambled mimic (Mimic negative control #1; CN001000-01), a miRIDIAN hairpin inhibitor for miR634 (IH-300961-03), a scrambled miRIDIAN hairpin inhibitor (negative control; IN-001005-01) and transfection controls (miRIDIAN mimic with Dy547; CP004500-01/miRIDIAN hairpin inhibitor with Dy547; IP-004500-01) were obtained from Dharmacon (Epsom, U
1
TochemistryA miRIDIAN mimic for miR-634 (C-300961-01), a scrambled mimic (Mimic negative control #1; CN001000-01), a miRIDIAN hairpin inhibitor for miR634 (IH-300961-03), a scrambled miRIDIAN hairpin inhibitor (negative control; IN-001005-01) and transfection controls (miRIDIAN mimic with Dy547; CP004500-01/miRIDIAN hairpin inhibitor with Dy547; IP-004500-01) were obtained from Dharmacon (Epsom, U
1
TochemistryA miRIDIAN mimic for miR-634 (C-300961-01), a scrambled mimic (Mimic negative control #1; CN001000-01), a miRIDIAN hairpin inhibitor for miR634 (IH-300961-03), a scrambled miRIDIAN hairpin inhibitor (negative control; IN-001005-01) and transfection controls (miRIDIAN mimic with Dy547; CP004500-01/miRIDIAN hairpin inhibitor with Dy547; IP-004500-01) were obtained from Dharmacon (Epsom, U
1
TochemistryA miRIDIAN mimic for miR-634 (C-300961-01), a scrambled mimic (Mimic negative control #1; CN001000-01), a miRIDIAN hairpin inhibitor for miR634 (IH-300961-03), a scrambled miRIDIAN hairpin inhibitor (negative control; IN-001005-01) and transfection controls (miRIDIAN mimic with Dy547; CP004500-01/miRIDIAN hairpin inhibitor with Dy547; IP-004500-01) were obtained from Dharmacon (Epsom, U
1
TochemistryA miRIDIAN mimic for miR-634 (C-300961-01), a scrambled mimic (Mimic negative control #1; CN001000-01), a miRIDIAN hairpin inhibitor for miR634 (IH-300961-03), a scrambled miRIDIAN hairpin inhibitor (negative control; IN-001005-01) and transfection controls (miRIDIAN mimic with Dy547; CP004500-01/miRIDIAN hairpin inhibitor with Dy547; IP-004500-01) were obtained from Dharmacon (Epsom, U
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Nology, Amsterdam, The Netherlands), according to the manufacturer's instructions. The RNA was hybridized with the LNA-based capture probe set (Exiqon, Vedbaek, Denmark) version 10 (annotation version 13). This probe set consists of 1344 probes including 725 human miRNAs, which are spotted in duplicate. Spots were quantified with the Imagene software (BioDiscovery), obvious outliers were removed a
1
Nology, Amsterdam, The Netherlands), according to the manufacturer's instructions. The RNA was hybridized with the LNA-based capture probe set (Exiqon, Vedbaek, Denmark) version 10 (annotation version 13). This probe set consists of 1344 probes including 725 human miRNAs, which are spotted in duplicate. Spots were quantified with the Imagene software (BioDiscovery), obvious outliers were removed a
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Ange was calculated. The microarray expression data has been deposited to the Gene Expression Omnibus (GEO) data repository (accession number GSE54665).RT-PCR3*104, for TOV112D 6*104 and for primary ovarian cell cultures 4*104 in a final volume of 450 L medium without antibiotics. On the day after seeding, 50 L of a mixture of Dharmafect 1 (final concentration 0.3 (v/v)) and mimic (final concent