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Pf and there was a small rise in expression between 10 hpf and 22 hpf (Figure 1(b)); the highest level was achieved at 22 hpf. kcnj2-12 gene expression decreased between 36 hpf and 48 hpf and then increased to its highest level after 48 hpf; peak expression was achieved at 72 hpf. The kcnj2 expression profile may correspond to skeletal muscle development in zebrafish. At 16 hpf, somite formation i
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Pf and there was a small rise in expression between 10 hpf and 22 hpf (Figure 1(b)); the highest level was achieved at 22 hpf. kcnj2-12 gene expression decreased between 36 hpf and 48 hpf and then increased to its highest level after 48 hpf; peak expression was achieved at 72 hpf. The kcnj2 expression profile may correspond to skeletal muscle development in zebrafish. At 16 hpf, somite formation i
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Pf and there was a small rise in expression between 10 hpf and 22 hpf (Figure 1(b)); the highest level was achieved at 22 hpf. kcnj2-12 gene expression decreased between 36 hpf and 48 hpf and then increased to its highest level after 48 hpf; peak expression was achieved at 72 hpf. The kcnj2 expression profile may correspond to skeletal muscle development in zebrafish. At 16 hpf, somite formation i
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D reported in GenBank (KC473502). Spatial and temporal expression profiling of the kcnj212 gene transcript was determined by quantitative realtime PCR (qRT-PCR) and whole mount in situ hybridisation (WISH). qRT-PCR was used to investigate the spatial expression of kcnj2-12 in adult zebrafish and the temporal expression in young embryos. In adult zebrafish, the kcnj212 gene is expressed primarily i
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D reported in GenBank (KC473502). Spatial and temporal expression profiling of the kcnj212 gene transcript was determined by quantitative realtime PCR (qRT-PCR) and whole mount in situ hybridisation (WISH). qRT-PCR was used to investigate the spatial expression of kcnj2-12 in adult zebrafish and the temporal expression in young embryos. In adult zebrafish, the kcnj212 gene is expressed primarily i
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Did not have an entry in the Zv9 database) and is located at approximately 40.40 Mb. The locations of the other four putative zebrafish transcripts are shown in Supplementary Figure 1 with the immediate flanking genes shown for each one. Only kcnj2-3 #1 is flanked by zebrafish orthologues of ccdc137 and btbd17. Based on the conservation of synteny, kcnj2-3 #1 and kcnj2-12 appear to be zebrafish co
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Uman KCNJ2. These two transcripts will be further investigated while the remaining three genes were not studied further. Primers spanning the entire coding region of the kcnj2-3 #1 and kcnj2-12 transcripts were designed to confirm the sequences as well as the expression of these genes in the zebrafish heart. No PCR amplicons were obtained using the kcnj2-3 #1 primers, suggesting that the gene may
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Lashing motion at 24 hpf [34]. This series of events coincide with the peak that is seen in the kcnj2-12 temporal profile between 10 hpf?2 hpf and could be the reason for the increase in the expression of this gene. To investigate the spatial expression of kcnj2-12 in young embryos, WISH was performed on embryos aged 24 hpf and 48 hpf. Two probes targeting the kcnj2-12 transcript showed similar ex